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Showing: 801 - 840 of 8763 results

  1. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  2. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

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  3. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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  4. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

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  5. pJK565

    Plasmid
    #72454
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72454-plasmid-map-sequence-id-133894

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  6. pJK578

    Plasmid
    #72457
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72457-plasmid-map-sequence-id-133895

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  7. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  8. pJK585

    Plasmid
    #72458
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72458-plasmid-map-sequence-id-133896

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  9. pTF257 (pFA6a-TEV-6xGly-His3MX)

    Plasmid
    #44068
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44068-plasmid-map-sequence-id-61660

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  10. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid
    #44062
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44062-plasmid-map-sequence-id-61661

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  11. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid
    #44080
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44080-plasmid-map-sequence-id-61664

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  12. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid
    #44074
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44074-plasmid-map-sequence-id-61665

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  13. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid
    #44092
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44092-plasmid-map-sequence-id-61662

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  14. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Expression
    Bacterial
    Promoter
    PosmY, PsulA, and grpE
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

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  24. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid
    #203348
    Purpose
    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203348-plasmid-map-sequence-id-399143

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  26. mCherry-ER-3

    Plasmid
    #55041
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55041-plasmid-map-sequence-id-173833

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  29. mApple-Alpha-V-Integrin-25

    Plasmid
    #54866
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54866-plasmid-map-sequence-id-330493

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  30. mCherry-Golgi-7

    Plasmid
    #55052
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55052-plasmid-map-sequence-id-352458

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  32. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

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  35. mCherry-CD9-10

    Plasmid
    #55013
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD9 (CD9 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55013-plasmid-map-sequence-id-234553

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  36. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  37. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available Since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105921-plasmid-map-sequence-id-443051

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  38. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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Showing: 801 - 840 of 8763 results