We narrowed to 580 results for: Abra

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p3xFLAG CMV14 / BRAF

Plasmid

#131710

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B N133I

Plasmid

#89449

Purpose

Expression of GFP-tagged human Rab27B N133I in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B N133I (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

N133I

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGImC_V5-CSNK1A1

Plasmid

#92014

Purpose

Expresses V5-CSNK1A1, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT_KCNQ3

Plasmid

#173163

Purpose

To generate Flp-In CHO-KCNQ3 stable cells

Depositor

Al George

Article

Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

Plasmid

#164827

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

Plasmid

#201120

Purpose

RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

TurboRFP; H2B-RFP

Use

Lentiviral

Mutation

No

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

Plasmid

#113693

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 gRNA Cassete

Use

AAV and CRISPR

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP304-pAAV-EFS-dSaCas9-VP64-pA

Plasmid

#113679

Purpose

A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Synthetic, Human)

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2557-2853)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-R438E

Plasmid

#91984

Purpose

Expresses FLAG-HA-AGO2 (R438E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

R438E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP2_AS

Plasmid

#74241

Purpose

Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47-VP2_AS

Use

AAV

Tags

19 alanines fused to VP2 of AAV9.47

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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natMX-ins7

Plasmid

#195044

Purpose

pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-WT

Plasmid

#91986

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

Plasmid

#184381

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Cas9, two gRNAs targeting dystrophin

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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