We narrowed to 10,372 results for: ESP

1. PC1575

   Plasmid

   #232088

   Purpose

   Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

   Depositor

   Pranam Chatterjee

   Article

   Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

   Insert

   uAb with cloning Esp3I restriction site
   

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME TAEL2.0-5xC120-minpro (JDW 1109)

   Plasmid

   #224507

   Purpose

   Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TAEL2.0-5xC120
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

   Plasmid

   #170195

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Promoter

   CMV51

   Available Since

   Nov. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166010

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

   Plasmid

   #166011

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV51

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

   Plasmid

   #69582

   Purpose

   Expresses p53 tagged with mKate2 and split N-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - N term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

   Plasmid

   #226102

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only encodes for residues 142-515 of DELE1 corres…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

   Plasmid

   #69585

   Purpose

   Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53-L344A (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   p53 L344A mutation that prevents tetramerization …

   Promoter

   EF1alpha

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

    Plasmid

    #170201

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV51

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid

    #69587

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

    Plasmid

    #170200

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    S13I, W152C, L452R, D614G

    Promoter

    CMV51

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid

    #69586

    Purpose

    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents dimerization and…

    Promoter

    EF1alpha

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Availability

    Academic Institutions and Nonprofits only

    

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15. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid

    #170196

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

    Plasmid

    #170197

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

    Promoter

    CMV51

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid

    #170198

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

    Plasmid

    #170188

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

    Promoter

    CMV51

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDest-557

    Plasmid

    #161890

    Purpose

    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 18065-M35-412

    Plasmid

    #225670

    Purpose

    Lentiviral protein expression of a TGF-beta pathway reporter

    Depositor

    Dominic Esposito

    Insert

    dsEGFP
    

    Use

    Lentiviral

    Promoter

    S3x6-JunBp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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