We narrowed to 747 results for: PI

1. PB-LacI-eGFP

   Plasmid

   #213021

   Purpose

   piggyBac donor vector containing LacI-eGFP for random integration by PBase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   LacI**-eGFP
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-n1-AICD

   Plasmid

   #69925

   Purpose

   mammalian expression of human AICD fused to EGFP

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   AICD (APP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   47 aa long

   Promoter

   CMV

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TetR-tdTomato

   Plasmid

   #212958

   Purpose

   piggyBac donor vector containing TetR-tdTomato for random integration by PBase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   TetR-tdTomato
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)mGAT1-0-GFP

   Plasmid

   #41662

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal hydrophobic isoleucine residue added a…

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3646

   Plasmid

   #137714

   Purpose

   Expresses LbCas12a RVRR variant in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   LbCas12a-RVRR
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R, K595R

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HeFSpCas9

    Plasmid

    #92355

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-dHeFSpCas9

    Plasmid

    #92116

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    Cbh

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-C1 SNX9 KR mutant K267N R327N

    Plasmid

    #161994

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K267N R327N

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126778

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Use

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)mGAT1YFP*

    Plasmid

    #41665

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; C-terminal hyd…

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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