Addgene

We narrowed to 684 results for: PRK5

Showing: 401 - 420 of 684 results

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pRK5-mEGFP-RUNX1-MUT

Plasmid

#194575

Purpose

Mammalian Expression of mEGFP-RUNX1 Mutant (G363Afs*229) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

G363Afs*229

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS (GPR155) ∆Permease-HA

Plasmid

#199686

Purpose

Transient expression of LYCHOS (GPR155) ∆Permease

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion from amino acid 1-369

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PRAS40 T246A

Plasmid

#15479

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Thr246 to Ala

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-FOXL2-MUT

Plasmid

#194585

Purpose

Mammalian Expression of mEGFP-FOXL2 Mutant (A328Pfs*28) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A328Pfs*28

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(L91P)

Plasmid

#99726

Purpose

Mammalian expression of Flag-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

L91P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(K84T)

Plasmid

#99727

Purpose

Mammalian expression of Flag-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

Flag

Expression

Mutation

K84T

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(R37P)

Plasmid

#99717

Purpose

Mammalian expression of Flag-RagA(R37P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

R37P

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc Rheb Omp25

Plasmid

#112764

Purpose

express myc-Rheb-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

myc-Rheb-Omp25 (RHEB Human)

Use

Tags

Omp25 and myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN (C terminus)

Plasmid

#72296

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SZT2 1-1021

Plasmid

#87036

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1-V242A

Plasmid

#33048

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

V242A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-TEAD1-I247A

Plasmid

#33049

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

I247A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-Flag CASTOR2 ACT1

Plasmid

#84493

Purpose

overexpression of CASTOR2 ACT1 domain (aa 1-171)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 ACT1 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-171 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag CASTOR1 ACT1

Plasmid

#84491

Purpose

overexpression of CASTOR1 ACT1 domain (aa 1-169)

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 ACT1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-169 amino acid

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (CTD)

Plasmid

#112768

Purpose

express GFP-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (C terminal domain only) (RRAGC Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (CTD)

Plasmid

#112769

Purpose

express Flag-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (C terminal domain only) (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (CTD)

Plasmid

#112771

Purpose

express Flag-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (C terminal domain only) (RRAGC Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagB (CTD)

Plasmid

#112766

Purpose

express GFP-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (C terminal domain only) (RRAGB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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HA GST deltaCT S6K1 pRK5

Plasmid

#8468

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta CT p85 s6k1 (Rps6kb1 Rat)

Use

Tags

ha/gst

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only