We narrowed to 14,014 results for: TIM

1. human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

   Plasmid

   #39306

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E211A, K212A, E213A, R214A, D215A and Y217A

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

   Plasmid

   #39307

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T249A, D250A and E251A

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209652

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Mutation

   A95K

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209653

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209654

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

   Plasmid

   #244072

   Purpose

   Cytosolic expression of green glucose sensor with non-responsive mIRFP

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2.mIRFP670nano3
   

   Use

   AAV

   Tags

   mIRFP670nano3

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.(ER).iGlucoSnFR2.HaloTag

   Plasmid

   #244073

   Purpose

   ER expression of green glucose sensor with non-responsive HaloTag

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2.HaloTag
   

   Use

   AAV

   Tags

   Calreticulin and HaloTag

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.CAG.(mem).iGlucoSnFR2

   Plasmid

   #244077

   Purpose

   Plasma membrane targeted expression of green glucose sensor

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2
   

   Use

   AAV

   Tags

   IgG kappa leader

   Available Since

   Sept. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244074

    Purpose

    ER expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    Calreticulin and HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244070

    Purpose

    Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.TBG.(cyto).iGlucoSnFR2.mIRFP670nano3

    Plasmid

    #244103

    Purpose

    Cytosolic expression of green glucose sensor with non-responsive mIRFP

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.mIRFP670nano3
    

    Use

    AAV

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET.HaloTag.v7

    Plasmid

    #226197

    Purpose

    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209663

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Mutation

    S29W.A95K

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209661

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    GFAP

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209666

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209657

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209665

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET.HaloTag.v7.AviTag

    Plasmid

    #195486

    Purpose

    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7.AviTag
    

    Tags

    6xHIS and AviTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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