We narrowed to 4,867 results for: gne

1. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_ACTB CRISPIE

   Plasmid

   #172853

   Purpose

   Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Inserts

   Actb intron 1 sgRNA
   DRS-2 sgRNA
   CRISPIE designer exon (phase 0-0) encoding mEGFP
   mRuby3
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234954

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pScalps_Puro_mTet2 catalytic domain

   Plasmid

   #79554

   Purpose

   Expression of catalytic domain of mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   aa990-1912

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pOEM1_pCMV:bmp4-pPH:vsvged

   Plasmid

   #111157

   Purpose

   Baculovirus rescue vector, VSVGED pseudotype, hShh

   Depositor

   Elly Tanaka

   Article

   Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

   Insert

   hBmp4 (BMP4 Human)
   

   Use

   Baculoviral rescue shuttle vector

   Tags

   Expression

   Insect and Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-2TKN-bp1

   Plasmid

   #133428

   Purpose

   Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   SREBF1 (SREBF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SREBP1a amino acids 1-50 fused to GST

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19_HDRT_TRAC_CD19-CAR

    Plasmid

    #183473

    Purpose

    Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

    Inserts

    TRAC (TRAC Human)
    CD19-specific CAR transgene insert
    TRAC (TRAC Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM30-ARC105

    Plasmid

    #133426

    Purpose

    Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 5-78 fused to C-terminal of …

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOEM1_pCMV:deltaN-bmp4-pPH:vsvged

    Plasmid

    #111158

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

    Insert

    deltaNhBmp4 (BMP4 Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    delta N, mutant BMP lacking the N terminus (delta…

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Bovine, Mouse)
    TRAC-3' homology arm (TRAC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-HDRT-CD3z-truncCAR(anti-CD19)

    Plasmid

    #215758

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR (CD247, CD19 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETCON-IgG1/design_50

    Plasmid

    #30301

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    IgG Fc Designed Binder IgG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pETCON-HB20/design_70

    Plasmid

    #30254

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB20
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 10, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pETCON-HB28/design_25

    Plasmid

    #30260

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB28
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pETCON-E43/design_55

    Plasmid

    #30237

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    Mtb ACP2 designed binding protein E43
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only