We narrowed to 428 results for: ipsc

1. pAAVS1-Neo-TNNT2-Blast

   Plasmid

   #214014

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-Neo-TNNT2-Hygro

   Plasmid

   #214015

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-Blast-TNNT2-Zeo

   Plasmid

   #214018

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLYBL-TO-PAX7

   Plasmid

   #204346

   Purpose

   Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   human PAX7
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)

   Plasmid

   #205097

   Purpose

   fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

   Insert

   mNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

   Plasmid

   #205098

   Purpose

   Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

   Insert

   mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJWK_VD_04

   Plasmid

   #158105

   Purpose

   CaV1.3 5'UTR luciferase reporter

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR of CACNA1D (uc003dgu.5)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJWK_VD_03

   Plasmid

   #158104

   Purpose

   CaV1.2 5'UTR luciferase reporter

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR of CACNA1C (uc009zdu.1)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Hygro-iRFP670

    Plasmid

    #99377

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CAG-ChR2-YFP

    Plasmid

    #204345

    Purpose

    PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    ChR2-YFP
    

    Tags

    Yellow fluorescent protein

    Expression

    Mammalian

    Mutation

    C-terminus (aa315-737) is deleted - PMID: 1461559…

    Promoter

    CAG

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJWK_VD_01

    Plasmid

    #158102

    Purpose

    5'UTR stem-loop luciferase reporter: 0kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop 0kcal/mol
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJWK_VD_02

    Plasmid

    #158103

    Purpose

    5'UTR stem-loop luciferase reporter: -30kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop -30kcal/mol
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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