We narrowed to 13,053 results for: SHI

1. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX PLCg1(NC)-SH2

   Plasmid

   #46471

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(NC) (PLCG1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 542-759

   Promoter

   Tac

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Use

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHLSec2-irisin-his

   Plasmid

   #122729

   Purpose

   Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Mouse, Bovine)
   

   Use

   Tags

   his and signal sequence for secretion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GNAS_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176976

   Purpose

   Mammalian expression vector encoding GNAS and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GNAS (GNAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCXN2-FLAG-AT2R-CFP

   Plasmid

   #101660

   Purpose

   Expresses AT2R with FLAG tag and CFP in mammalian cells.

   Depositor

   Yusuke Ohba

   Article

   Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

   Insert

   Angiotensin II type 2 receptor (AGTR2 Human)
   

   Use

   Tags

   ECFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-Flag-Ago2

   Plasmid

   #72207

   Purpose

   Mammalian expression of full-length human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Grb2-SH2

   Plasmid

   #46440

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grb2 (GRB2 Human)
   

   Use

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 58-159

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-FKBP-MAVS(pLxIS)

   Plasmid

   #221256

   Purpose

   Expression of MAVS pLxIS (393-441) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS pLxIS (393-441) (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GABPA_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176970

    Purpose

    Mammalian expression vector encoding GABPA and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GABPA (GABPA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PX458-sgTSC2

    Plasmid

    #128098

    Purpose

    CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    sgRNA against human TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BII-ChBtW-dnTCF4-ires-GFP

    Plasmid

    #133378

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    dnTCF4 (TCF4 Human)
    

    Use

    Tags

    2x Flag

    Expression

    Mammalian

    Mutation

    deltaN31

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-TASL-NEMO

    Plasmid

    #221267

    Purpose

    Expression of TASL-NEMO IKK binding site in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    fusion of TASL with NEMO IKK binding site (Tasl, Ikbkg Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-TASL-PLPLR

    Plasmid

    #221266

    Purpose

    Expression of TASL-STING PLPLR in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    fusion of TASL with STING PLPLR (Sting1, Tasl Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GNAS_pLENTI-CAG-IRES-GFP

    Plasmid

    #176991

    Purpose

    Mammalian lentiviral expression vector encoding GNAS

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GNAS (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWPI-LUC7L-3xFLAG

    Plasmid

    #201640

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L (LUC7L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. fck-Jaws-GFP-ER2

    Plasmid

    #65013

    Purpose

    Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-GFP-ER2
    

    Use

    Lentiviral

    Tags

    ER2 and GFP

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223-ZNF706

    Plasmid

    #88037

    Purpose

    Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF706 (ZNF706 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSYC-190

    Plasmid

    #178962

    Purpose

    Expresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cells

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Insert

    Glial fibrillary acidic protein (GFAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R79C

    Promoter

    CMV IE94 Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRCN0000338399

    Plasmid

    #78156

    Purpose

    shXPO1 targeting CDS

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    hU6 and hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pDONR223-ZNF692

    Plasmid

    #88701

    Purpose

    Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF692 (ZNF692 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hsyn-Jaws-GFP-ER2

    Plasmid

    #65016

    Purpose

    AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-GFP-ER2
    

    Use

    AAV

    Tags

    ER2 and GFP

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid

    #197576

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311G, C313A

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BAF-WT-tet-puro

    Plasmid

    #199648

    Purpose

    Doxycycline-inducible BAF expression

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    BAF (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX Grb2 SH2-SH3

    Plasmid

    #46442

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 1-217

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcdna6.2- myc ULK1 4SA

    Plasmid

    #27631

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCV-MAVS(IRSp53 pLxIS)

    Plasmid

    #221315

    Purpose

    Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-FKBP-STING(pLxIS)

    Plasmid

    #221240

    Purpose

    Expression of STING CTT in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FOR human OCT4 TALEN

    Plasmid

    #52412

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD NG NN NN NN HD NG HD NG HD HD HD NI NG
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

32. p-miR-Report + WT miR-181 BS in GATA6 3UTR

    Plasmid

    #22794

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

33. p-miR-Report + MT miR-181 BS in GATA6 3UTR

    Plasmid

    #22795

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CL20_mEGFP_DOC2B_USP43

    Plasmid

    #205806

    Purpose

    Express mEGFP-tagged fusion protein, DOC2B_USP43 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    DOC2B_USP43 (DOC2B, USP43 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR4-hDND1

    Plasmid

    #70070

    Purpose

    Gateway entry vector for human DND1 with stop codon

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (DND1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLX311 HRAS

    Plasmid

    #117749

    Purpose

    Open reading frame vector encoding HRAS

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    RASH1 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Closed vector so will not read through the V5 reg…

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSCV-mouse Gfi1-IRES-GFP

    Plasmid

    #91891

    Purpose

    Retroviral expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV gag

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX Abl-SH2

    Plasmid

    #46402

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Abl (ABL1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 120-225

    Promoter

    Tac

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GLI1_pLENTI-CAG-IRES-GFP

    Plasmid

    #176992

    Purpose

    Mammalian lentiviral expression vector encoding GLI1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. fck-Jaws-KGC-GFP-ER2

    Plasmid

    #65012

    Purpose

    Lentivirus-mediated expression of Jaws under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    Lentiviral

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GLI1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176968

    Purpose

    Mammalian expression vector encoding GLI1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid

    #176589

    Purpose

    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Chesapeake Bay) locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated CRISPR array to two direct repeats, rep…

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. BAF-S4A-tet-puro

    Plasmid

    #199649

    Purpose

    Doxycycline-inducible BAF expression

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    BAF (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S4A

    Promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. BAF-S4D-tet-puro

    Plasmid

    #199650

    Purpose

    Doxycycline-inducible BAF expression

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    BAF (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S4D

    Promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDONR223-ZNF770

    Plasmid

    #88562

    Purpose

    Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF770 (ZNF770 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLVCT-Dnd1-EGFP

    Plasmid

    #106343

    Purpose

    Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1-EGFP (Dnd1 Synthetic, Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid

    #184409

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQCXIN- myc ULK1 wt

    Plasmid

    #27626

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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