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MBP-GTSE1(long)-His6

Plasmid

#69110

Purpose

For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

Depositor

Stephen Royle

Publication

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Tags

His6 and MBP

Expression

Bacterial

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-AK1

Plasmid

#31290

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

adenylate kinase 1 (AK1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/GS-DJ1-D149A-V5

Plasmid

#29345

Depositor

Mark Cookson

Publication

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Asp149Ala

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCR8-DJ1-WT

Plasmid

#29354

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-GALK1

Plasmid

#23743

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GALK1 (GALK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Publication

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BCL2L1_WT

Plasmid

#82193

Purpose

Gateway Donor vector containing BCL2L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BCL2L1 (BCL2L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Cofilin-N-10

Plasmid

#54048

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Cofilin (CFL1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Publication

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (HV.1)

Plasmid

#241116

Purpose

Expresses SARS-CoV-2 spike (HV.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (HV.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,Q52H,V83A,G142D,Y1…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (EG.5)

Plasmid

#241114

Purpose

Expresses SARS-CoV-2 spike (EG.5) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (EG.5) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I, L24del, P25del, P26del, A27S, V83A, G142D, …

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-LAP3

Plasmid

#217660

Purpose

For gateway cloning of LAP3

Depositor

Karla Feijs

Publication

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

LAP3 (LAP3 Human)

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA1

Plasmid

#201626

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA2

Plasmid

#201627

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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hIgG1_heavy_CoVMab7

Plasmid

#194605

Purpose

Mammalian expression of CoVMab7 heavy chain

Depositor

Ben Murrell

Insert

CoVMab7 heavy chain (S )

Expression

Mammalian

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F1

Plasmid

#184163

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Publication

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

Plasmid

#157984

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Publication

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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