We narrowed to 8,640 results for: dykddddk
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Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT19
Plasmid#223391PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.DepositorInsertAtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT27
Plasmid#223399PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.DepositorInsert2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT13
Plasmid#223385PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.DepositorInsertAtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdest N-FLAG EGFP puro
Plasmid#223056PurposeEGFP gene expression in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5 FRT TO FLAG LRRK1
Plasmid#232062PurposeExpresses FLAG-LRRK1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLRRK1 (LRRK1 Human)
TagsFLAGExpressionMammalianAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-SpdCas9-Dnmt3A/3L-WT
Plasmid#222497PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 WT
Plasmid#231585PurposeALPK1 WT gene under control of a doxycycline-inducible promoterDepositorAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 T237M
Plasmid#231586PurposeALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged threonine 237 to MethioninePromoterdoxycyclineAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-Jarid2-polyA
Plasmid#223811PurposeIVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.DepositorInsertJarid2 (Jarid2 Mouse)
UseIn vitro transcriptionTagsFlagExpressionMammalianPromoterCMV, T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP
Plasmid#203965PurposeDox inducible FLAG tag ARID1a in a sleeping beauty transposon constructDepositorInsertARID1A (Arid1a Mouse)
UseSleeping beauty transposonTagsFLAGExpressionMammalianPromoterTREAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-L2HGDH(opt)-FLAG
Plasmid#230089Purposeexpressing human L2HGDHDepositorInsertL2HGDH (L2HGDH Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV_CMV_FLAG-VSVG
Plasmid#212642PurposeLentivirus vector to express FLAG-VSVGDepositorInsertVSVG
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-CGBE1
Plasmid#198537PurposeExpresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
BmTyr-Flag
Plasmid#220779Purposenon-targeted BmTyr throughout the cytoplasmDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBacillus megaterium Tyrosinase
TagsFlag tagExpressionMammalianPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human RNF130-3X FLAG
Plasmid#225331PurposeLentiviral expression of human RNF130 with 3x FLAG tag in mammalian cellsDepositorAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG005_Spike_Inducible_3xFlag
Plasmid#191578PurposeInducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseLentiviralTags3x FLAGExpressionMammalianPromoterTRE3GAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-CIP2A
Plasmid#222623Purposemammalian expression vector of Flag-tagged CIP2ADepositorAvailable sinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits