We narrowed to 9,314 results for: Coli

1. pSCL.108

   Plasmid

   #184977

   Purpose

   Express -Eco1 LYP1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   LYP1 donor E27stop

   Promoter

   Gal7

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSCL.106

   Plasmid

   #184975

   Purpose

   Express -Eco1 CAN1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   CAN1 donor G444stop

   Promoter

   Gal7

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCL.72

   Plasmid

   #184984

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker1-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.95

   Plasmid

   #184986

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker2-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLS.544

   Plasmid

   #184961

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   murF donor G1359A, a1/a2 length extended to 22 bp

   Promoter

   T7/lac

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCL.037

   Plasmid

   #184966

   Purpose

   Express -Eco1 dead RT and ncRNA in yeasst

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Human codon optimized RT, YXDD catalytic region m…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSCL.112

   Plasmid

   #184981

   Purpose

   Express -Eco1 FAA1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FAA1 donor P233stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSCL.002

    Plasmid

    #184972

    Purpose

    Express -Eco1 ADE2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCL.110

    Plasmid

    #184979

    Purpose

    Express -Eco1 TRP2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TRP2 donor E64stop

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRB116.2

    Plasmid

    #181946

    Purpose

    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    TorR-mNG; mCherry; tetR (AX04_RS23485, torR torR - E. coli, Synthetic)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRB120.2

    Plasmid

    #181947

    Purpose

    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 torR - E. coli, Synthetic)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLS.407

    Plasmid

    #187204

    Purpose

    Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v32
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLS.408

    Plasmid

    #187205

    Purpose

    Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB_EA_3EGFP

    Plasmid

    #185967

    Purpose

    Expresses EGFP from PR promoter in E.coli Dh5alpha cell

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Pr-Egfp cassette in forward direction in Network Brick
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PR

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-ammB3

    Plasmid

    #167815

    Purpose

    Expresses His6-tagged AmmB3 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmB3
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-ammC1

    Plasmid

    #167816

    Purpose

    Expresses His6-tagged AmmC1 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmC1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFE-sfGFP

    Plasmid

    #162688

    Purpose

    pFE vector control expressing sfGFP

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    sfGFP
    

    Use

    Tags

    C terminal 6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge