We narrowed to 4,243 results for: Pet2

1. Met1 diUb (1-152, R42W)

   Plasmid

   #110767

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   R42W

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. cryS96

   Plasmid

   #67519

   Purpose

   αB-crystallin minipeptide fused to ELP (S96).

   Depositor

   Andrew MacKay

   Article

   Wang et al J Control Release. 2014 Oct 10;191:4-14.

   Insert

   mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
   

   Use

   Tags

   elastin-like polypeptide (ELP) S96

   Expression

   Bacterial

   Mutation

   mini-αB-crystallin contains residues 73–92 of aB-…

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HisGB1-Hif1a(776-826)_FIH

   Plasmid

   #99508

   Purpose

   Bacterial expression of hydroxylated Hif1a TAD (776-826)

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   FIH1 (HIF1AN Human)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MEK1C218

   Plasmid

   #164639

   Purpose

   Bacterial expression plasmid for His6-MEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. DHFR-C-HiBiT

   Plasmid

   #207119

   Purpose

   Bacterially expressed DHFR-HiBiT for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   Human-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
   

   Use

   Tags

   10xHis-tag and HiBiT tag

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. *MtbCM-HiBiT-His

   Plasmid

   #228028

   Purpose

   Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag

   Depositor

   James Inglese

   Article

   van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71

   Insert

   Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
   

   Use

   Tags

   10xHis-tag with thrombin site and HiBiT Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. *MtbCM-bio-His

   Plasmid

   #186027

   Purpose

   Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71

   Insert

   Rv1885c Chorismate Mutase
   

   Use

   Tags

   Avi-tag for in vitro biotinylation and His-tag wi…

   Expression

   Bacterial

   Mutation

   Codon optimization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8R6A

   Plasmid

   #228446

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   DTX1 (DTX1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-hMICU1EF

    Plasmid

    #199180

    Purpose

    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

    Depositor

    Ming-Feng Tsai

    Article

    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Tags

    His6 and MBP, Thrombin site, TEV site

    Expression

    Mutation

    D421A, E432K, F433W, F453W

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. His6-EGFP-cPHx1

    Plasmid

    #183674

    Purpose

    PtdIns(3,4)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 169-329

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MEK1C218C222

    Plasmid

    #164640

    Purpose

    Bacterial expression plasmid for His6-MEK1C218C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S218C/S222C/C277S/C376S

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mature ISG15 C-term. Domain (79-165, W123A)

    Plasmid

    #110765

    Purpose

    Bacterial expression of mutant proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    proISG15 C-term. domain (79-165, W123A) (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    W123A

    Promoter

    T7

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. DOT1L-CC1

    Plasmid

    #111756

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    contains coiled coil #1 (residues 506-555)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. CCR5-SZ218a QTY

    Plasmid

    #162444

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. CCR5-SZ190b QTY

    Plasmid

    #162445

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRIP12-V2_A755-A841

    Plasmid

    #194759

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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