We narrowed to 66,069 results for: des.1

Showing: 4201 - 4220 of 66069 results

Results per page:

Sort results by:

pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
GFP-FAK WT

Plasmid

#186148

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ITSN-mcherry-sspB

Plasmid

#85220

Purpose

Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

ITSN1(1225-1575) (ITSN1 Human)
mcherry
sspB

Tags

NA

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EBFP2-Gamma-Tubulin-17

Plasmid

#55242

Purpose

Localization: Centrosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

R57H in Gamma Tubulin

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pClneoMyc human CIN85

Plasmid

#47935

Purpose

expresses Myc tagged human CIN85 in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

CIN85 (SH3KBP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 2-665 of human CIN85; D114G

Promoter

CMV

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Cx43-7

Plasmid

#55023

Purpose

Localization: Gap Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Cx43 (Gja1 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc rat S-SCAM alpha

Plasmid

#40213

Depositor

Yutaka Hata, Yoshimi Takai

Article

Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

Insert

S-SCAM (Magi2 Rat)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-puro GFP-p62 D337, 338, 339A

Plasmid

#38280

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed D337A, D338A, D339A

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-PDPK1

Plasmid

#23801

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDPK1 (PDPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T-Rac1-Q61L

Plasmid

#12979

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-OptoFGFR1

Plasmid

#59776

Purpose

Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1 (FGFR1 Human, Mustard Weed)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OpenCRISPR-1 sgRNA-12nt stem

Plasmid

#221567

Purpose

Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 12nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Expression

Mammalian

Mutation

wt

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-FOG1[N+C]

Plasmid

#100085

Purpose

dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

FOG1(aa 1-45) (ZFPM1 Human)

Use

CRISPR

Tags

3XFLag-NLS-FOG1-dCas9-FOG1-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b- hTPI

Plasmid

#50723

Purpose

overexpression of human TPI1 in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge