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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 38,499 results for: LAT;

Showing: 4201 - 4220 of 38499 results

  1. pBiFC-PBZ-4A-VC

    Plasmid
    #110649
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Use
    Tags
    HA and VC
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110649-plasmid-map-sequence-id-217742

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  6. ITGB1

    Plasmid
    #51920
    Purpose
    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB1 (ITGB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 728, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51920-plasmid-map-sequence-id-79898

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  8. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid
    #210567
    Purpose
    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210567-plasmid-map-sequence-id-417655

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  12. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Article
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Phage, Human, Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40591-plasmid-map-sequence-id-302078

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  13. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid
    #69113
    Purpose
    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to confer shRNA resistance.
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69113-plasmid-map-sequence-id-121841

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  16. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  18. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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Showing: 4201 - 4220 of 38499 results