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pFastBacDual with LFn + FlaAAA

Plasmid

#84867

Purpose

Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
FlaAAA

Use

Baculovirus

Tags

6xHis

Expression

Insect

Mutation

3 C-terminal leucines have been replaced by alani…

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein N-ter

Plasmid

#73520

Purpose

Encodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein N-ter (residues 1-373) (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Sense

Plasmid

#60638

Purpose

Expresses the human EPHB4 exon 11/12 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre2_60.F2

Plasmid

#60210

Purpose

Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Insert

Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)

Tags

FKBP12(F36V) and Nuclear localisation signal

Expression

Mammalian

Mutation

FKBP12 has the F36V mutation; amino acids 60-343 …

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + T356

Plasmid

#58911

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T356 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S780

Plasmid

#58915

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S780 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S788

Plasmid

#58916

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S788 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S807

Plasmid

#58918

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S807 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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EYFP-Abr

Plasmid

#36414

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EYFP

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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Reporter

Plasmid

#36008

Depositor

Larry Gerace

Article

Ohba et al J Cell Biol. 2004 Dec 20;167(6):1051-62.

Insert

LAP2β-TM

Use

Reporter

Tags

11kD FRB and EGFP

Expression

Mammalian

Mutation

FRB is residues 2025-2114 of FRAP, LAP2β-TM is re…

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
LCV2 STING KO

Plasmid

#217445

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting STING (STING1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 heavy chain

Plasmid

#198251

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-FCHO1

Plasmid

#196910

Purpose

visualization of clathrin-coaed vesicle formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

FCHO1 (FCHO1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Promoter

U6

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBsCas12a

Plasmid

#174671

Purpose

Expresses 6xHis-MBP tagged BsCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBsCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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