We narrowed to 34,848 results for: CaS;

1. FnoCas12a-2C-NLS in pCSDest

   Plasmid

   #126639

   Purpose

   Expresses FnoCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. OA-1050E (Ubiq-CasRx)

   Plasmid

   #132416

   Purpose

   Express CasRx under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   CasRx
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ubiq

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-CIB1-dCas9

   Plasmid

   #63665

   Purpose

   Mammalian gRNA expression vector also expressing CIB1-dCas9

   Depositor

   Dong Kong

   Article

   Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_075 AsCas12a D908A

   Plasmid

   #171668

   Purpose

   For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a D908A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 908 to alanine

   Promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaSpeR-act5cB-GAL4QF

   Plasmid

   #61309

   Purpose

   Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   GAL4_DBD::QF2w_AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed EK on C-terminus of QF2 to KKKK

   Promoter

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1807_pmax-dCasRx-FKBP

   Plasmid

   #118640

   Purpose

   Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   dCasRx-FKBP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 Cas9-POLD3

   Plasmid

   #183197

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-POLD3
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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