162,660 results

1. p13xQUAS-ZsGreen-P2A, α-cry:mCherry

   Plasmid

   #180480

   Purpose

   Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   13x QUAS-ZsGreen-P2A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. CF-Inp(D281A)

   Plasmid

   #20156

   Depositor

   Tobias Meyer

   Article

   Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

   Insert

   CFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Stop codon at position 331. D281A mutation.

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hACE2

   Plasmid

   #145033

   Purpose

   Express human ACE2 with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   human ACE2 (ACE2 Human)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. EGFP-Rab7A Q67L

   Plasmid

   #28049

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   Available since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v6-10 (fl)

   Plasmid

   #137820

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length

   Promoter

   PGK

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE_puro

   Plasmid

   #118692

   Purpose

   Empty vector to express overexpression cDNA

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

   Plasmid

   #163047

   Purpose

   GCaMPer calcium sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   GCaMPer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_3xFLAG-ATP6V0A3-mScarlet

   Plasmid

   #228867

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IZ-ANXA11-mRuby3

    Plasmid

    #184894

    Purpose

    Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R230C relative to NP_001148.1

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSNAPf-H2B Control Plasmid

    Plasmid

    #101124

    Purpose

    Expression of SNAP-tagged Histone H2B in mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p11d-tRPA(123)

    Plasmid

    #102613

    Purpose

    Expression of human RPA in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Inserts

    RPA1 (RPA1 Human)
    RPA2 (RPA2 Human)
    RPA3 (RPA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS_12x-601_MA1

    Plasmid

    #114358

    Purpose

    12x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    12X601-MA1
    

    Use

    Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIVT-p53DD

    Plasmid

    #232751

    Purpose

    IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    p53DD
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    deletion of DNA binding domain

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA_RFP-TEVprotease

    Plasmid

    #124947

    Purpose

    Expression of RFP-TEVprotease

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-TEVprotease
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EphA2-YFP

    Plasmid

    #108852

    Purpose

    Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Tags

    Labeled with eYFP on the C-Terminus via a 15 amin…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Geminin-mStrawberry reporter

    Plasmid

    #158690

    Purpose

    This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166017

    Purpose

    mammalian expression of HCoV-NL63 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-NL63 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MSCV-Cas9-2A-BFP

    Plasmid

    #164662

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    Cas9
    BFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-FLAG LAP2

    Plasmid

    #15738

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LAP2 (CEBPB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. cyto-Ruby3-iATPSnFR1.0

    Plasmid

    #102551

    Purpose

    expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    Ruby3-iATPSnFR1.0
    

    Use

    Tags

    Ruby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBbA2c-RFP

    Plasmid

    #35326

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SpdCas9 VPR

    Plasmid

    #188509

    Purpose

    Expresses FLAG tagged SpdCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge