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Showing: 6331 - 6360 of 167150 results

  8. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  13. pLenti-AncBE4max

    Plasmid
    #174164
    Purpose
    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.
    Depositor
    Wensheng Wei
    Article
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Insert
    AncBE4max
    Use
    Lentiviral
    Tags
    P2A-mCherry
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174164-plasmid-map-sequence-id-342437

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  17. pYPQ265E9

    Plasmid
    #213472
    Purpose
    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tadde-32aa linker-zspc…
    Tags
    NO
    Expression
    Plant
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213472-plasmid-map-sequence-id-426728

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  19. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

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  28. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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Showing: 6331 - 6360 of 167150 results