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pMXs-uORFx6-Isl1-P2A-mRuby2

Plasmid

#233174

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mLhx3-P2A-mRuby2

Plasmid

#233175

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFwx1-mLhx3-P2A-mRuby2

Plasmid

#233176

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx1-mLhx3-P2A-mRuby2

Plasmid

#233177

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx2-mLhx3-P2A-mRuby2

Plasmid

#233178

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx3-mLhx3-P2A-mRuby2

Plasmid

#233179

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx6-mLhx3-P2A-mRuby2

Plasmid

#233180

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-mNgn2x3HA-P2A-mRuby2

Plasmid

#233157

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Isl1-P2A-mRuby2

Plasmid

#233158

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-mLhx3-P2A-mRuby2

Plasmid

#233159

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Isl1-T2A-mLhx3

Plasmid

#233160

Purpose

Retroviral expression of Isl1 and Lhx3 for motor neuron specification

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-T2A-mLhx3 (Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mNgn2x3HA-T2A-mLhx3

Plasmid

#233162

Purpose

Retroviral expression of mNgn2x3HA and Lhx3

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-T2A-Lhx3 (Neurog2, Isl1 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mNgn2x3HA-P2A-mRuby2

Plasmid

#233163

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2

Plasmid

#233164

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx1-mNgn2x3HA-P2A-mRuby2

Plasmid

#233165

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2

Plasmid

#233166

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2

Plasmid

#233167

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mNgn2

Plasmid

#233148

Purpose

Retroviral expression of Ngn2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Isl1

Plasmid

#233149

Purpose

Retroviral expression of Isl1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1 (Isl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mLhx3

Plasmid

#233150

Purpose

Retroviral expression of Lhx3

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mBrn2

Plasmid

#233151

Purpose

Retroviral expression of Brn2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mAscl1

Plasmid

#233152

Purpose

Retroviral expression of Ascl1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-NIL

Plasmid

#233154

Purpose

Retroviral expression of Ngn2, Isl1, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

NIL (Isl1, Lhx3, Neurog2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-mBrn2-P2A-mRuby2

Plasmid

#233156

Purpose

Retroviral expression of fluorescent mRuby2 reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Brn2-2A-mRuby2 (Pou3f2 Synthetic, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 Neo sgAGAP1_3

Plasmid

#233248

Purpose

Knock out of murine AGAP1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

AGAP1 (Agap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 Neo PTEN sgRNA2

Plasmid

#233245

Purpose

Knock out of murine PTEN

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PTEN (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 Neo sgAGAP1_2

Plasmid

#233247

Purpose

Knock out of murine AGAP1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

AGAP1 (Agap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-KLF2-T2A-mCHERRY

Plasmid

#224295

Purpose

Expresses mouse KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TBET-T2A-mCHERRY

Plasmid

#224296

Purpose

Expresses mouse TBET and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

TBET T2A mCherry (Tbx21 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-ER-KLF2-T2A-mCHERRY

Plasmid

#224297

Purpose

Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Estrogen Receptor

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

Plasmid

#233065

Purpose

Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Leucine 928 to Alanine; Methionine 929 to Alanine

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

Plasmid

#233064

Purpose

Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

Plasmid

#233063

Purpose

Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Threonine 923 to Glycine

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3

Plasmid

#233062

Purpose

Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

Alfa and HiBiT

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1B1-3

Plasmid

#228811

Purpose

Expression of mouse EHMT1 with deletion of aa 1185-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1185-1298

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1B1-4

Plasmid

#228812

Purpose

Expression of mouse EHMT1 with deletion of aa 809-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 809-1298, See Depositor Comments

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP_EHMT1V09

Plasmid

#228813

Purpose

Expression of mouse EHMT1 with deletion of aa 1-31

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1-31

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VirTREX2-HLDel-1_NbmiR164e/h

Plasmid

#231156

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/h

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbmiR164e/h

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VirTREX2-HLDel-1_NbATML1-2pro and NbATML1-1pro

Plasmid

#231154

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VirTREX2-HLDel-1_SlMIR164b

Plasmid

#231152

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting SlMIR164b

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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