We narrowed to 17,027 results for: puromycin

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pPBpuro-LDR

Plasmid

#173857

Purpose

Encoding a part of the rapamycin-induced dimerization system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

a Lyn11-targeted FRB (LDR)

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMP472

Plasmid

#134997

Purpose

Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Use

AAV and Synthetic Biology

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMP498

Plasmid

#134998

Purpose

Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

Use

AAV and Synthetic Biology

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Egr2ASRNA

Plasmid

#177737

Purpose

Egr2 AS-RNA lentiviral expression vector

Depositor

Nikolaos Tapinos

Article

Martinez-Moreno et al Cell Rep. 2017 Aug 22;20(8):1950-1963. d

Insert

Egr2-AS-RNA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S11A-puro

Plasmid

#44112

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 11 to Alanine

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) cRel shRNA2

Plasmid

#22510

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA2 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pLX302-NIPP1-V5 puro

Plasmid

#87775

Purpose

Expresses V5 tagged human NIPP1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

NIPP1 (PPP1R8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_2

Plasmid

#36350

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
nSR100L4

Plasmid

#35174

Depositor

Benjamin Blencowe

Article

Calarco et al Cell. 2009 Sep 4;138(5):898-910.

Insert

MmnSR100

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLEX-FHH-Raf1RBD

Plasmid

#120707

Purpose

Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Raf1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Raf1 amino acids 1-149

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
lentiCRISPR - C3orf17 sgRNA 1

Plasmid

#70652

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against C3orf17

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only