We narrowed to 2,603 results for: ubiquitin

1. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

   Plasmid

   #203803

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q68L

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-CDK11B-V5-miniTurbo_IDG-K

   Plasmid

   #189914

   Purpose

   Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK11B (CDK11B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-USP13

   Plasmid

   #61812

   Purpose

   to make USP13 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

   Plasmid

   #203802

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJOG022

   Plasmid

   #105384

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor Zea mays Ubiquitin (1500bp)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI/ BpiI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 ISG15

   Plasmid

   #67054

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol1-Spe-KpnI-ubb-nmKate

   Plasmid

   #238373

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVUT-eC3GFP

    Plasmid

    #225485

    Purpose

    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251, See Depositor Comments

    Promoter

    Human ubiquitin C

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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