161,873 results

1. AAV-U6gRNA1-U6gRNA2-TnT-Cre

   Plasmid

   #87682

   Purpose

   AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

   Depositor

   William Pu

   Article

   Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

   Inserts

   gRNA1
   gRNA2
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   U6 and cTnT

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEos3.2-C1

   Plasmid

   #54550

   Purpose

   Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. HA-HIF1alpha P402A/P564A-pBabe-puro

   Plasmid

   #19005

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKb-TnpB2

   Plasmid

   #215334

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Use

   Tags

   Not applicable

   Expression

   Plant

   Mutation

   Promoter

   Rice ubiquitin promoter for TnpB and maize ubiqui…

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/7

   Plasmid

   #112863

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap7
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-1 TIM9,10

   Plasmid

   #170280

   Purpose

   Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

   Insert

   mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rictor_1 shRNA

   Plasmid

   #1853

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV/TO GFP-Zeo DEST (719-1)

   Plasmid

   #17431

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-DuraPETase

   Plasmid

   #218699

   Purpose

   Encodes codon optimized DuraPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DuraPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPR63-DuET

    Plasmid

    #213289

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR63 (GPR63 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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