We narrowed to 5,629 results for: pcas

1. p2T-CMV-BE4maxCP1028-BlastR

   Plasmid

   #152993

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9 CP1028-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

   Plasmid

   #222993

   Purpose

   Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAYP (pDre)

   Plasmid

   #62709

   Purpose

   Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   Dre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ-176

   Plasmid

   #195424

   Purpose

   Transient, constitutive expression of synZiFTR: ZF1-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-CDAmax-BlastR

   Plasmid

   #152995

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   CDA-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CRY2PHR-PSD95FingR-GFP

   Plasmid

   #89443

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-PSD95FingR-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains CRY2 a.a. 1-498

   Promoter

   CAG

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGJJ045

   Plasmid

   #183621

   Purpose

   abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

   Depositor

   Ben Lehner

   Article

   Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Use

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHEE901

   Plasmid

   #91707

   Purpose

   CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR023_PIE-PUM2

    Plasmid

    #204819

    Purpose

    Expression PIE-PUM2 fusion protein in mammalian cells

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    Pumilio 2 (PUM2 Human)
    

    Use

    Tags

    HA-Flag-rApobec1 and huADAR2dd

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NPM1_mNeonGreen_Donor

    Plasmid

    #178093

    Purpose

    HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1-mNeonGreen HDR donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

    Plasmid

    #222992

    Purpose

    Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LMNA_mScarlet-I_Donor

    Plasmid

    #178092

    Purpose

    HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA-mScarlet-I HDR donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRANS_211

    Plasmid

    #91110

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge