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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 70,757 results for: LAS

Showing: 6391 - 6420 of 70757 results

  1. pDUAL_2_araBAD

    Plasmid
    #155176
    Purpose
    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155176-plasmid-map-sequence-id-310563

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  3. pYPQ230-E795L

    Plasmid
    #176890
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    LbCas12a-E795L
    Use
    CRISPR; Gateway compatible lbcpf1 entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176890-plasmid-map-sequence-id-354003

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  8. pV92.2

    Plasmid
    #78409
    Purpose
    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
    Depositor
    Andrew Fire
    Article
    Silas et al Science. 2016 Feb 26;351(6276):aad4234.
    Inserts
    RT-Cas1
    Cas2
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    78409-plasmid-map-sequence-id-146291

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  14. AAV-SYN-eGFP-Kindlin 1

    Plasmid
    #203733
    Purpose
    AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    Fermt1 (Fermt1 Mouse)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203733-plasmid-map-sequence-id-402640

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  16. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lac
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53286-plasmid-map-sequence-id-83099

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  18. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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  25. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

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  28. AAV-SYN-TdTomato-RAB11

    Plasmid
    #203732
    Purpose
    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203732-plasmid-map-sequence-id-402639

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Showing: 6391 - 6420 of 70757 results