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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13,583 results for: TIM

Showing: 10651 - 10700 of 13583 results

  1. pDIV490

    Plasmid
    #177698
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177698-plasmid-map-sequence-id-352588

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  6. pCEP4-SERTS585P-myc

    Plasmid
    #107498
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S585P
    Promoter
    CMV
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107498-plasmid-map-sequence-id-205777

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  10. p.UTA.2.0-GL-2

    Plasmid
    #82440
    Purpose
    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2
    Depositor
    Jens Gruber
    Article
    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mutation
    The complementary region for GL2 siRNA control wa…
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82440-plasmid-map-sequence-id-168133

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  11. pMP650

    Plasmid
    #135000
    Purpose
    VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Use
    Lentiviral and Synthetic Biology
    Tags
    DpnI(aa146-254), V5 tag, and VP-64
    Expression
    Mammalian
    Mutation
    DpnI truncated to aa146-254
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135000-plasmid-map-sequence-id-267928

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  28. As3 (pBW411J117-arsR)

    Plasmid
    #123142
    Purpose
    An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123142-plasmid-map-sequence-id-241980

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  41. pCEP4-SERTG484E-myc

    Plasmid
    #107471
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G484E
    Promoter
    CMV
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107471-plasmid-map-sequence-id-205611

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  42. pCEP4-SERTS477F-myc

    Plasmid
    #107470
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S477F
    Promoter
    CMV
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107470-plasmid-map-sequence-id-205590

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  49. pCEP4-SERTI425V-myc

    Plasmid
    #107464
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    I425V
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107464-plasmid-map-sequence-id-205573

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  50. pMM549

    Plasmid
    #112531
    Purpose
    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23107 and ProD
    Available since
    Feb. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    112531-plasmid-map-sequence-id-217579

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Showing: 10651 - 10700 of 13583 results