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We narrowed to 5,960 results for: actin

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Showing: 4261 - 4280 of 5960 results

  2. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  5. pLexM-HsPQLC1(Δ100-125/GGGS)-FLAG

    Plasmid
    #250422
    Purpose
    Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker) in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Δ100-125 (Replaced with GGGS)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    250422-plasmid-map-sequence-id-499042

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  6. pLexM-HsPQLC1(Δ100-125/3xGGGS)-FLAG

    Plasmid
    #250424
    Purpose
    Expression of C-terminally FLAG-Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a 3xGGGS Linker) in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Δ100-125 (Replaced with 3xGGGS)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    250424-plasmid-map-sequence-id-499019

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  7. pX462-ABE8e-nSaCas9

    Plasmid
    #237462
    Purpose
    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    3xFlag_ABE8e_SaCas9 -6xNLS
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    chicken beta-actin promoter & U6
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237462-plasmid-map-sequence-id-476147

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  11. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

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  13. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  15. pLexM-HsPQLC1(16-271)-TEV-GFP-His8

    Plasmid
    #250419
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-15), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-15 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250419-plasmid-map-sequence-id-499018

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  16. pLexM-HsPQLC1(20-271)-TEV-GFP-His8

    Plasmid
    #250420
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-19), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-19 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250420-plasmid-map-sequence-id-499015

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  17. pLexM-HsPQLC1(24-271)-TEV-GFP-His8

    Plasmid
    #250421
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-23), HsPQLC1 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ1-23 (new Start codon added)
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250421-plasmid-map-sequence-id-498996

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  18. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222015-plasmid-map-sequence-id-457427

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  19. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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Showing: 4261 - 4280 of 5960 results