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pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-NUCr

Plasmid

#61169

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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NET97 pmRFP-N2 (641)

Plasmid

#61994

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

AADACL1 (NCEH1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
TAL2473

Plasmid

#36854

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Right (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2304

Plasmid

#36733

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Left (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2246

Plasmid

#36691

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PHF8-TALEN_Left (Phf8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1098

Plasmid

#29029

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple202 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1096

Plasmid

#29027

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple200 (SLC6A5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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SULT4A1

Plasmid

#25480

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SULT4A1 (SULT4A1 Human)

Tags

His

Expression

Bacterial

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Publication

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
XP1 Xwnt-1

Plasmid

#16990

Depositor

Randall Moon

Publication

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-1 (wnt1.L Frog)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBS/KS-mId1sp

Plasmid

#16043

Depositor

Robert Benezra

Publication

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id1 (Id1 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 390-742

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-701/+114)-Luc

Plasmid

#13409

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
siRNA-resistant pLV-SigmaR1-mNeonGreen

Plasmid

#226569

Purpose

Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)

Depositor

Gia Voeltz

Publication

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Lentiviral

Tags

mNeonGreen

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-mGFP-SNX9

Plasmid

#223632

Purpose

Lentiviral expression of human SNX9 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SNX9 (SNX9 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-EGFP-CAAX

Plasmid

#208685

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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