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pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

Plasmid

#70197

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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HL 2818 (=HL 865 + pHL 489)

Plasmid

#30032

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pTU3-B-RFP

Plasmid

#72946

Purpose

Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - B site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HL 4218 (=HL 716 + pHL 1411)

Plasmid

#30105

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

T7noRBS, GFP

Expression

Bacterial

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
HL 4212 (=HL 716 + pHL 1414)

Plasmid

#30104

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

T7noRBS, GFP

Expression

Bacterial

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
HL 839 (=HL 716 + pHL 108)

Plasmid

#30008

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pHL600

Plasmid

#37563

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

CsrB (csrB E. coli)
CsrA (csrA E. coli)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only
HL 2312 (=HL 716 + pHL 787)

Plasmid

#30024

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
HL 3858 (=HL 716 + pHL 1304)

Plasmid

#30088

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 2817 (=HL 716 + pHL 720)

Plasmid

#30031

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:mCherry, pLlacO-1:sodB::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 4120 (=HL 716 + pHL 1370)

Plasmid

#30094

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 2305 (=HL 716 + pHL 765)

Plasmid

#30020

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 2308 (=HL 716 + pHL 814)

Plasmid

#30022

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pTipi2.2a

Plasmid

#252899

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, N and C-term tags

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Insert

Constitutive GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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ED_p_044b

Plasmid

#246072

Purpose

ECOR31 mCpol D57A bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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p028g13-6His

Plasmid

#249634

Purpose

Clone 6xHis-residues in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Insert

N-terminal tag with 6x His-residues

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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p028g13-8His

Plasmid

#249635

Purpose

Clone 8xHis-residues in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Insert

N-terminal tag with 8x His-residues

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pA_higher-CheY-pMag-mNeonGreen

Plasmid

#253260

Purpose

Encodes the fusion of CheY, pMag, and mNeonGreen driven with the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

CheY_pMag_mNeonGreen

Tags

pMag and mNeonGreen

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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