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eGFP-pk-S65A

Plasmid

#115619

Purpose

Expresses human Parkin S65A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

S65A

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-DCAMKL2

Plasmid

#23604

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCAMKL2 (DCLK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
p153 pPr-cre

Plasmid

#8403

Purpose

Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

probasin promoter

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJA1

Plasmid

#177655

Purpose

Recombinant protein synthesis of human DNAJA1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJA1 (DNAJA1 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_07_01

Plasmid

#165014

Purpose

Expression of HLA-DRB1*07:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*07:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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p7140 pHAGE-P-CMVt-N-HA-GAW-EME1

Plasmid

#100150

Purpose

Expresses EME1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EME1 (EME1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Erik Rodriguez, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_HuD

Plasmid

#65763

Purpose

expresses GFP-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta

Plasmid

#64676

Purpose

expresses Wild type WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223-DKFZp761P0423

Plasmid

#23475

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DKFZp761P0423 (PRAG1 Human)

Use

Gateway Cloning

Mutation

GS inserted after aa343, del aa1068-1184

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#222742

Purpose

CaST-IRES for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Human, Synthetic)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ENO1-Halo

Plasmid

#175330

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ENO1 (ENO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-alpha1AR

Plasmid

#106064

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-alpha1AR

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cdt1 human

Plasmid

#72681

Purpose

mammalian expression of human Cdt1

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdt1 (CDT1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only