We narrowed to 29,111 results for: PLE;
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Plasmid#213292PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR84 (GPR84 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGES401
Plasmid#190198PurposeFor CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-C Med12His
Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-hATG2A
Plasmid#114462PurposeExpresses human ATG2A with GFP in mammalian cellsDepositorInserthuman ATG2A (ATG2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationP656R (please see depositors comment below)PromoterCMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP
Plasmid#55642PurposesCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Scre-dependentTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianMutationPromoterCMV/hUBCAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XMS2 Lentiviral Vector
Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AExpressionMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPR87-DuET
Plasmid#213293PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR87 (GPR87 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
UseTags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Cep290
Plasmid#27380DepositorInsertCep290 (Cep290 Mouse)
UseTagsmCherryExpressionMammalianMutationplease see depositor comments belowPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
UseTags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
GPR123-DuET
Plasmid#213262PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR123 (ADGRA1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE8e-V106W
Plasmid#193646PurposeExpression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationTadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR77-DuET
Plasmid#213291PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR77 (C5AR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR146
Plasmid#106047PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)DepositorInsertOpto-GPR146 (GPR146 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-M1R
Plasmid#106069PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)DepositorInsertOpto-M1R (CHRM1 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPR146-DuET
Plasmid#213265PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR146 (GPR146 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-XRCC1-EGFP-Hygro
Plasmid#176062PurposeEGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassetteDepositorInsertX-ray repair cross complementing 1 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-A2AR
Plasmid#106066PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)DepositorInsertOpto-A2AR (ADORA2A Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
Plasmid#35509PurposeAAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorHas ServiceAAV5 and AAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only