164,313 results

1. p3XFlag-CAPN1

   Plasmid

   #60941

   Purpose

   Expresses calpain1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain1 (CAPN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

   Plasmid

   #162377

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   7mer insertion in the AAV2 cap

   Promoter

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX puro TRIM21-GFP

   Plasmid

   #116941

   Purpose

   Expression of TRIM21-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   TRIM21 - GFP (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Blast-mStayGold

   Plasmid

   #227251

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-N-His

   Plasmid

   #119755

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   10xHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1 T2 CRIPR in pX330

   Plasmid

   #72833

   Purpose

   CRISPR/Cas to target the AAVS1 locus in human cells

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Cas9 and gRNA for targeting the AAVS1 locus in human cells
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. JNK-KTR-mStrawberry reporter

    Plasmid

    #158689

    Purpose

    This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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