We narrowed to 28,942 results for: Tat

Showing: 8641 - 8680 of 28942 results

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AAV-CAGGS-mNAGA

Plasmid

#196448

Purpose

Expresses modified α-N-acetylgalactosaminidase in mammalian cells

Depositor

Yuichiro Miyaoka

Article

Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

Insert

modified alpha-N-acetylgalactosaminidase (NAGA Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1899)

Plasmid

#170143

Purpose

Expresses residues 1-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1900-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1547)

Plasmid

#170144

Purpose

Expresses residues 1-1547 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1548-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (K999R)

Plasmid

#170142

Purpose

Expresses CHD7 (K999R) in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Mutation

K999R

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-455

Plasmid

#26319

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-455 (MIR455 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

Plasmid

#47453

Purpose

epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (N136,Grm2,C63)-cib1

Use

AAV; Tale

Tags

HA

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom0.6

Plasmid

#87353

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

0.6 kbp proximal promoter of human MCT2 (nested d…

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn11C3S-EGFP

Plasmid

#202747

Purpose

weakly plasma membrane targeted fluorescent protein

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

LYN (1-11) (C3S):EGFP (LYN Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V E37G

Plasmid

#12277

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282kB mut-LUC

Plasmid

#46867

Purpose

murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

NF- kB site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pLV Reep4-V5

Plasmid

#175120

Purpose

Lentiviral expression of mouse Reep4-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep4 (Reep4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (D380N)

Plasmid

#31248

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Aspartic acid 380 changed to Asparagine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282C/EBP mut-LUC

Plasmid

#46868

Purpose

murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

CEBP site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-LplA(AAG)

Plasmid

#61820

Purpose

Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

GFP

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2

Plasmid

#197117

Purpose

Expression of Myc-tagged PSK2 / TAOK1 in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-499

Plasmid

#26320

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-499 (MIR499A Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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D5_Ld_280600

Plasmid

#135821

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

major surface protease gp63, putative,leishmanolysin, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1MycHis/HIP1/ΔT

Plasmid

#31251

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Tags

his and myc

Expression

Bacterial

Mutation

deletion of TALIN region

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-shAUF1 hairpin 1

Plasmid

#52921

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pD765 HMGCR 3'UTR

Plasmid

#105766

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

HMGCR 3'UTR (HMGCR Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_S214C

Plasmid

#51077

Purpose

mammalian expression of ARAF S214C

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

S214C

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA1

Plasmid

#192304

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA1 (KPNA1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Mus.1

Plasmid

#196681

Purpose

Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

WVLPSGG insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MDV1B

Plasmid

#196680

Purpose

Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

KTVGTVY insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-D319N

Plasmid

#40820

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

D319N

Promoter

CMV

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pMAL-c2X-ORF24-NTD

Plasmid

#138465

Purpose

Expresses MBP-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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hisSUMO-Gli1

Plasmid

#87225

Purpose

Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

GLI1 (Gli1 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLV V5-nurim

Plasmid

#175110

Purpose

Lentiviral expression of V5-tagged mouse Nrm

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Nrm (Nrm Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ORF24-NTD

Plasmid

#138464

Purpose

Expresses GST-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-Fab1 (Francisella tularensis)

Plasmid

#61690

Purpose

Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

Depositor

Michael Johnson

Article

Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

Insert

enoyl-[acyl-carrier-protein] reductase (NADH)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-ORF24-3xFLAG

Plasmid

#138423

Purpose

Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

Plasmid

#67648

Purpose

I-SceI cleavage site at lys1-hphMX6

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

lys1 (lys1 Fission Yeast)
hphMX6

Use

To test i-scei cleavage efficiency

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-MeCP2_3

Plasmid

#36379

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MeCP2 (MECP2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PPP1CB C3.5 gRNA

Plasmid

#90845

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CB (Guide Designation C3.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 28,942 results for: Tat