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We narrowed to 6,088 results for: cat.2

Showing: 4321 - 4340 of 6088 results

  1. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  2. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  3. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

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  5. pAMPK alpha2 delta312X

    Plasmid
    #60127
    Purpose
    expresses constitutively active AMPKalpha2
    Depositor
    Morris Birnbaum
    Insert
    AMPK alpha 2 (Prkaa2 Rat)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Truncated at residue 312
    Promoter
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60127-plasmid-map-sequence-id-317039

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  10. HOXC5 (human) HIS-tag pET

    Plasmid
    #8563
    Depositor
    Corey Largman
    Insert
    HOXC5 (HOXC5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Starts at Amino Acid #2 (Ser); makes protein by W…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  19. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    David Root, John Doench
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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Showing: 4321 - 4340 of 6088 results