We narrowed to 6,524 results for: sas

1. pEBTetBl-CLIP-CPAP

   Plasmid

   #136864

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-CENPJ (CENPJ Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA180

   Plasmid

   #216023

   Purpose

   Fragmid fragment: (Cas protein) NGRR(T) PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTet-SNAP-CPAP

   Plasmid

   #136815

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CENPJ (CENPJ Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TS-PE2-C-WPRE

   Plasmid

   #176650

   Purpose

   Expresses C-terminus of PE2 in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Inserts

   C-terminus of PE2
   Artificial SA
   WPRE
   bGH poly(A)
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   None

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINTO-NSA::hSNRNP70

   Plasmid

   #61726

   Purpose

   Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   SNRP70 (SNRNP70 Human)
   

   Tags

   mSA (monomeric streptavidin)

   Expression

   Mammalian

   Mutation

   Two silent mutations to ensure restriction site c…

   Promoter

   T-REx (CMV + 2xTetO2)

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINTO-NSA::mEzh2

   Plasmid

   #61725

   Purpose

   Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   Enhancer of zeste homolog 2 (Ezh2 Mouse)
   

   Tags

   mSA (monomeric streptavidin)

   Expression

   Mammalian

   Promoter

   T-REx (CMV + 2xTetO2)

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-dSa VP64 Actc1

   Plasmid

   #99691

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-dSa VP64 Neurog2

   Plasmid

   #99695

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Sept. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P1-Ataxin3Q22-UIM*

   Plasmid

   #22114

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   UIM 1, 2, 3 SA-DG

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

10. DG SaCas9 GFP V3

    Plasmid

    #226961

    Purpose

    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. DG SaCas9 puro V3

    Plasmid

    #226960

    Purpose

    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBTetDBl-CLIP-CPAP

    Plasmid

    #136865

    Purpose

    Mammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-CENPJ (CENPJ Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEBTetD-SNAP-CPAP

    Plasmid

    #136816

    Purpose

    Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CENPJ (CENPJ Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pONSY-Lifeact:mCherry

    Plasmid

    #111876

    Purpose

    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Lifeact fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJG01

    Plasmid

    #213505

    Purpose

    expresses TdTomato in Capsaspora owczarzaki

    Depositor

    Joseph Gerdt

    Insert

    TdTomato
    

    Promoter

    EF1

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJP118

    Plasmid

    #176494

    Purpose

    Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Insert

    Lifeact peptide
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pONSY

    Plasmid

    #111873

    Purpose

    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Type

    Empty backbone

    Use

    Capsaspora owczarzaki

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pONSY-Venus

    Plasmid

    #111875

    Purpose

    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge