We narrowed to 7,497 results for: val

Showing: 4321 - 4340 of 7497 results

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pAC152-dual-dCas9VP64-sgExpression

Plasmid

#48238

Purpose

Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA Tag, HA-Tag, and VP64

Expression

Mammalian

Mutation

D10A;H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Pansio

Plasmid

#214441

Purpose

BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-1

Plasmid

#184834

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-NT

Plasmid

#184835

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194284

Purpose

EF1a-dCas9-KRAB-GFP with nontarget sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI

Plasmid

#221218

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsGIGYF1_AI

Plasmid

#148961

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Mito-CanlonicSF/pCMV-myc-mito

Plasmid

#224465

Purpose

To explore mitochondrial lactate dynamics

Depositor

Alejandro San Martín

Article

Rauseo et al bioRxiv 2024.07.19.604361

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

Plasmid

#133282

Purpose

Lentiviral expression of a peroxisomal eGFP

Depositor

Etienne Sokal

Article

Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

Insert

Peroxisome targeting signal 1

Use

Lentiviral

Tags

Peroxisome targeting signal 1

Expression

Mammalian

Promoter

None

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLX317 SLC2A1

Plasmid

#193685

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2-VSV

Plasmid

#139344

Purpose

Expression of TYK2 protein

Depositor

Sandra Pellegrini

Article

Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pDest17_N-myc

Plasmid

#234045

Purpose

Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

Depositor

Tanja Mittag

Article

Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

Insert

N-myc (MYCN Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-RPE65

Plasmid

#206346

Purpose

AAV plasmid expressing RPE65 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

Insert

Rpe65 (Rpe65 Mouse)

Use

AAV

Mutation

450-Met (C57BL6 natural variation)

Promoter

1.7kb human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT003

Plasmid

#171628

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and EF1-a

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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