We narrowed to 4,380 results for: 1-Oct;

1. C513-E06: attB5r-pA-polyhedrinp>-attB1r

   Plasmid

   #162948

   Purpose

   Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV polyhedrin promoter with upstream polyA sequence
   

   Use

   Synthetic Biology

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

   Plasmid

   #162965

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Halotag fusion tag with upstream E. coli ribosome binding site
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

   Plasmid

   #162964

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

   Plasmid

   #162957

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

   Plasmid

   #162955

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

   Plasmid

   #162954

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGMKOSiE

   Plasmid

   #20865

   Depositor

   Keisuke Kaji

   Article

   Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

   Insert

   myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
   

   Expression

   Mammalian

   Available Since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-SLC22A1_STOP

   Plasmid

   #161200

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTol1-UAS:ChrimsonR-tdTomato

   Plasmid

   #124231

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   ChrimsonR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Promoter

   14xUAS-E1b

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid

    #162950

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    

    Use

    Synthetic Biology

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-PalmGRET

    Plasmid

    #158221

    Purpose

    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    PalmGFP-Nluc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

    Plasmid

    #128327

    Purpose

    Reporter to evaluate YAP1/TEAD-mediated gene transcription

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Inserts

    H2B mCherry
    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPC1-hVAP-A KD/MD

    Plasmid

    #104449

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed K94 to D and M96 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CBh-Alb-mNG

    Plasmid

    #183466

    Purpose

    To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-GRET

    Plasmid

    #158222

    Purpose

    Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    GFP-Nluc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQCXIP-mCherry-Halo-YAP1

    Plasmid

    #128336

    Purpose

    Retrovirus construct to express mCherry-Halo-YAP1

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry and Halo-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUW TetO HA-AMPKa2 WT

    Plasmid

    #69826

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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