We narrowed to 7,164 results for: 11

1. Chicken ArcLight A173

   Plasmid

   #53615

   Purpose

   Genetically encoded voltage sensor ArcLight with improved kinetics

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Chicken ArcLight-A173
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Gg-VSP contains an R153Q mutation and an amino ac…

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1 SV40-CMV-SaCas9-3xNLS

   Plasmid

   #78601

   Purpose

   AAV vector containing SaCas9

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   SV40-CMV-SaCas9-3xNLS
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40, CMV promoters

   Available since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pODN-mNG-RHOA

   Plasmid

   #183836

   Purpose

   Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA homology arms with mNeonGreen-linker (RHOA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1 UBXN2A

   Plasmid

   #113505

   Purpose

   Bacterial expression of GST-tagged UBXN2A

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   UBXN2A (UBXN2A Human)
   

   Use

   Tags

   GST-Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltraI-Tet3.0[TAA]

   Plasmid

   #164580

   Purpose

   Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mb Tet3.0RS (R2-3)
   Mm tRNA[TAA]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L170G, N311G, C313A

   Promoter

   ProK and TacI

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKII-GRAB_eCB2.0

   Plasmid

   #164608

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S383T

   Promoter

   CaMKII

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-UAP56

   Plasmid

   #157661

   Purpose

   Expresses UAP56 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Spliceosome RNA helicase DDX39B (DDX39B Human)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-43

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

   Plasmid

   #140198

   Purpose

   Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a(D156R)-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated, D156R for improved activ…

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

    Plasmid

    #78603

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SaDMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pCMX-PL1-WDR5

    Plasmid

    #59970

    Purpose

    Mammalian Expression of WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

12. H2B-mAvicFP1

    Plasmid

    #129511

    Purpose

    Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    H2B-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 fused to the C terminus of human histone…

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1.H1-NP

    Plasmid

    #134367

    Purpose

    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    H1-NP (HRH1 Human)
    

    Use

    Tags

    Flag and natural peptide of nanoluciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FH1-MGT#2-mCherry_PGK-H2B-CFP

    Plasmid

    #164102

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCol1a1-frt (FVB)

    Plasmid

    #63575

    Purpose

    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting; Frt/flpe

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    None

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES-hrGFP II-mTET1

    Plasmid

    #83569

    Purpose

    Expresses TET1 with a mutated catalytic domain in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    catalytic domain mutant H1672D, D1674A

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

    Plasmid

    #132686

    Purpose

    AAV plasmid encoding mouse ACY1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Acylase I (Acy1, Acy1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-Rab7a-7

    Plasmid

    #56443

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pODN-mR2-ACTB

    Plasmid

    #183868

    Purpose

    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB homology arms with mRuby2-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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