Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,230 results for: LucY

Clear keywords
Showing: 4341 - 4360 of 5230 results

  6. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid
    #52299
    Purpose
    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.
    Depositor
    Christopher A Walsh
    Article
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Insert
    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    Use
    Luciferase
    Mutation
    15-bp deletion in the promoter
    Promoter
    Human GPR56 e1m promoter
    Available Since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52299-plasmid-map-sequence-id-80926

    Enlarge

  8. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Synthetic, Human, Photinus pyralis)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

    Enlarge

  11. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

    Enlarge

  15. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168121-plasmid-map-sequence-id-332861

    Enlarge

  16. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

    Enlarge

  17. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

    Enlarge

  18. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-CuO
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232477-plasmid-map-sequence-id-471678

    Enlarge

  19. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

    Enlarge

Showing: 4341 - 4360 of 5230 results