We narrowed to 5,537 results for: PID

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pNIC28-SPRTN core (26-240)

Plasmid

#253402

Purpose

Expression in E. coli of SPRTN 26-240 truncation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

SPRTN (SPRTN Human)

Tags

His

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-UbcH5c-S22R

Plasmid

#253413

Purpose

Expression in E. coli of UBCH5C carrying the S22R mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

UBCH5C (UBE2D3 Human)

Tags

GST

Expression

Bacterial

Mutation

S22R

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ORF6-BirA

Plasmid

#253132

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), BirA fused to orf6, C-terminal

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD_ORF6-BirA (ORF6 SARS-CoV-2)

Tags

BirA

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_XBB_ΔE67 3*

Plasmid

#253122

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a, premature stop codon in orf3a at AA213. GFP is inserted in the locus of env

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔEΔ6Δ7 orf3*

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ΔE/GFP Δ68

Plasmid

#253121

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA) , orf6, orf8. GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_RABVG Δ67a

Plasmid

#253177

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; rabies glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with RABVG

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_Rho-tag-Pro30-mEGFP-NLS3x

Plasmid

#249671

Purpose

Expression of rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x

Plasmid

#249672

Purpose

Expression of silicon rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_amhc(myh6)_cmlc2-nmKate

Plasmid

#238375

Purpose

Drives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting amhc

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-cmlc2-nGFP-nCas9

Plasmid

#238308

Purpose

Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

Cas9 (Csn1) endonuclease from Streptococcus pyogenes

Use

Tol2-based plasmid

Tags

nGFP-P2A

Promoter

cmlc2 (myl7)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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KLF17-CRa1-Lsg-MS2

Plasmid

#247476

Purpose

sgRNA1 for KLF17 activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA1 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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KLF17-CRa2-Lsg-MS2

Plasmid

#247477

Purpose

gRNA2 for KLF17 activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS484_10xHis_MBP_TEV_dSpRY

Plasmid

#244832

Purpose

Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-B*07:02-BirA

Plasmid

#239752

Purpose

E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-B*07:02 (HLA-B Human)

Tags

BirA substrate peptide

Expression

Bacterial

Mutation

G120C

Promoter

T7

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK56K_3G7

Plasmid

#177301

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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