We narrowed to 5,034 results for: PID

1. pEGFP-imp α

   Plasmid

   #119718

   Purpose

   Expresses EGFP-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

   Insert

   importinα (KPNA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains amino acids 251-529

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PJ-DEAD

   Plasmid

   #38002

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1 (SAC1 Budding Yeast)
   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Asp 1263 mutated to Ala and Cys 779 mutated to Ser

   Promoter

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-membrane-mEGFP

   Plasmid

   #105526

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   GNAI2 (GNAI2 Human)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   fragment encoding the plasma membrane targeting s…

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

   Plasmid

   #190790

   Purpose

   [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Inserts

   Cas9
   mazF-LVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Mutation

   silent mutations of BpiI-sites in original Cas9 g…

   Promoter

   PnrsB and Ptrc-[E*](104)

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-GPAT4152-208

   Plasmid

   #134468

   Purpose

   GPAT4152-208 expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   GPAT4 (GPAT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GPAT4, amino acids 152-208

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZc-ScAct1*-thymosinB-8His

   Plasmid

   #111148

   Purpose

   Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   Act1 (ACT1 Budding Yeast)
   

   Use

   Pichia pastoris integration plasmid

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PJ-Sac

   Plasmid

   #38000

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1 (SAC1 Budding Yeast)
   INPP5E (INPP5E Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Asp 1263 mutated to Ala

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP-FKBP (YF)

   Plasmid

   #20175

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP (YF): (FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FKBP was taken out from pC4M-F2E with a flanking …

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2_EGFP

   Plasmid

   #155098

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0212

    Plasmid

    #142558

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCV2_mCherry

    Plasmid

    #155096

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIP mScarlet-ER [TM(SAC1)]

    Plasmid

    #186572

    Purpose

    Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LCV2_mClover3

    Plasmid

    #155095

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag_HsIRE1a_pBabePuro

    Plasmid

    #54337

    Purpose

    N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    The endogenous IRE1a signal sequence (aa1-18) was…

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP mScarlet-MOSPD2 RD/LD

    Plasmid

    #186476

    Purpose

    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-IRES-blast GFP-SPNS1

    Plasmid

    #198571

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PuroR-P2A-DD-TCOF1-donor

    Plasmid

    #60747

    Purpose

    Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

    Depositor

    Benhur Lee

    Article

    Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

19. BlastR-P2A-DD-TCOF1-donor

    Plasmid

    #60748

    Purpose

    Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

    Depositor

    Benhur Lee

    Article

    Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. H516 SONIC KrasG12D-IRES-luciferase donor

    Plasmid

    #138178

    Purpose

    CRISPR SONIC: Kras G12D luciferase donor plasmid.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    KrasG12D (Kras Mouse)
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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