We narrowed to 6,950 results for: SIM

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pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

Plasmid

#213034

Purpose

fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

Insert

mNeonGreen, ISN-IE, ChRmine

Use

AAV

Tags

Gaussia luciferase signal sequence

Promoter

hSyn

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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SB-SRT-Puro

Plasmid

#154888

Purpose

Sleeping Beauty self-reporting transposon with puromycin reporter

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

Sleeping beauty SRT

Use

Transposon

Expression

Mammalian

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

Plasmid

#221997

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Plasmid

#159799

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ARH2-c003

Plasmid

#173094

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRHL1 (ADPRHL1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-K354, codon optimized

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pQTEV-YWHAE

Plasmid

#31562

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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MDO2-c004

Plasmid

#173104

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K7-D243

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SI-112

Plasmid

#131127

Purpose

C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pOPS0263

Plasmid

#133228

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pOPS0262

Plasmid

#133227

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-pDUXCca

Plasmid

#198236

Purpose

Lentivector with tet-inducible porcine DUXC (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

porcine DUXC (codon altered)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN

Plasmid

#50519

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBC155

Plasmid

#202274

Purpose

Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc182

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC114

Plasmid

#202276

Purpose

Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc141

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB2791

Plasmid

#63654

Purpose

pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MTK0_051

Plasmid

#123948

Purpose

Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hAAVS1 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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