170,823 results
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Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only
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SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
TagsHis6 and TEV siteExpressionBacterialPromoterT5Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRTC2-puro
Plasmid#190567PurposeExpresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.DepositorInsertL1 (L1.3) with reporter cassette
ExpressionMammalianPromoterSV40Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512
Plasmid#161875PurposeExpresses Br512 polymerase in E.coliDepositorArticleInsertModified DNA Polymerase I Large Fragment (Br512)
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7, tetPAAvailable SinceNov. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 D210N
Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
TagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
ExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-YapS127A
Plasmid#46049DepositorInsertYes-kinase associated protein (YAP1 Human)
TagsFlag tagExpressionMammalianMutationS127APromoterEF1aAvailable SinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only