We narrowed to 34,855 results for: CaS;

1. V1-MESA-45F-M-dCas9

   Plasmid

   #84504

   Purpose

   MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V2-MESA-35F-M-dCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-MBP-huHkCas12a

   Plasmid

   #174683

   Purpose

   Expresses 6xHis-MBP tagged HkCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huHkCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

   Plasmid

   #173203

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCas9 PE2-3HA

   Plasmid

   #169853

   Purpose

   Mammalian Expression, SpyCas9 prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-M-MLV-3HA
   

   Use

   Tags

   3XHA tag and bpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-ArCas12a-2AeGFP

   Plasmid

   #123632

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   ArCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-LpCas12a-2AeGFP

   Plasmid

   #123635

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   LpCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASP-SptP120-TD-HilA

   Plasmid

   #153330

   Purpose

   Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV dCas9-MC (eGFP)

   Plasmid

   #89931

   Purpose

   Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deactivated Cas9, fragment of M.SssI (residues 27…

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459V2.0-SpCas9-HF4

   Plasmid

   #108295

   Purpose

   pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-dHeFSpCas9

    Plasmid

    #92116

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    Cbh

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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