We narrowed to 6,963 results for: RAP

1. pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

   Plasmid

   #58380

   Purpose

   tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   avi-EGFP-rpl10a
   

   Use

   Tags

   Avi Tag and EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMito-PAGFP-FRB.

   Plasmid

   #60005

   Purpose

   Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Type

   Empty backbone

   Use

   Knocksideways

   Tags

   FRB and PAGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRB-polySH35

   Plasmid

   #175234

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FRB-polySH33

   Plasmid

   #175233

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA-Nore1

   Plasmid

   #1979

   Depositor

   Joseph Avruch

   Article

   Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

   Insert

   Nore1 (Rassf5 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

6. FLAG-Nore1

   Plasmid

   #1975

   Depositor

   Joseph Avruch

   Article

   Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

   Insert

   Nore1 (Rassf5 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pJD427

   Plasmid

   #154371

   Purpose

   Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

   Depositor

   Joseph Davis

   Article

   Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

   Insert

   proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GST-Nore1

   Plasmid

   #1971

   Depositor

   Joseph Avruch

   Article

   Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

   Insert

   Nore1 (Rassf5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

9. MZB216

   Plasmid

   #221766

   Purpose

   "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   rtxA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FKBP-GFP-emerin

    Plasmid

    #222415

    Purpose

    To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    Emerin (EMD Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFOSGE_CUS1B08

    Plasmid

    #71087

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED7 (MED7 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pFOSGE_CUS1D07

    Plasmid

    #71085

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED10 (MED10 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pFOSGE_COF2G03

    Plasmid

    #70882

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    TfIIFalpha (TfIIFalpha Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRC_RR_VP1_r1c3

    Plasmid

    #65724

    Purpose

    Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

    Depositor

    Junghae Suh

    Article

    Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)

    Plasmid

    #163505

    Purpose

    DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIS GEM-CEPIA1er

    Plasmid

    #58217

    Purpose

    Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    GEM-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GEM-GECO1 E298D F359W D400E E407D

    Promoter

    CAG

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FKBP-NTEVp(WT)

    Plasmid

    #137831

    Purpose

    Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-NTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRB-CTEVp(WT)

    Plasmid

    #137832

    Purpose

    Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKM3

    Plasmid

    #154168

    Purpose

    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-BE-zCas9

    Plasmid

    #101739

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGAN230

    Plasmid

    #131122

    Purpose

    reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-Cerulean-Epac2-Venus

    Plasmid

    #138196

    Purpose

    FRET-based biosensor for monitoring Epac2 activation.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSK221

    Plasmid

    #129249

    Purpose

    reverse yTRAP, tetR-responsive Neon reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    pADH1(tetO2)-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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