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Plasmid#202155PurposeLevel 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertmTurquoise2 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_DE-RFP1
Plasmid#202154PurposeLevel 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertRFP1 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE-mRuby3
Plasmid#202153PurposeLevel 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertmRuby3 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_DE-3xStop
Plasmid#202151PurposeLevel 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.DepositorInsert3x Stop codon
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CD-eGFP
Plasmid#202149PurposeLevel 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-Venus
Plasmid#202148PurposeLevel 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertVenus gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CD-sfGFP
Plasmid#202147PurposeLevel 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertsfGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-mTurquoise2
Plasmid#202146PurposeLevel 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertmTurquoise2 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CD-mRFP1
Plasmid#202145PurposeLevel 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertmRFP1 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eYGFP-uv
Plasmid#202144PurposeLevel 0 plasmid containing an eYGFP-uv fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteYGFP-uv gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-mcherry
Plasmid#202143PurposeLevel 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertmCherry gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eCFP
Plasmid#202142PurposeLevel 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteCFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-roGFP
Plasmid#202140PurposeLevel 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertroGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eYFP
Plasmid#202139PurposeLevel 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteYFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AF-spacer
Plasmid#202130PurposeLevel 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.DepositorInsertSpacer
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
FKBP-TalinRod-mEmerald
Plasmid#202382PurposeFKBP12-Talin(434-2541)-mEmeraldDepositorInsertFKBP12-Talin(434-2541)-mEmerald
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-TalinC11-mEmerald
Plasmid#202381PurposeFKBP12-Talin(1974-2541)-mEmeraldDepositorInsertFKBP12-Talin(1974-2541)-mEmerald
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-TalinN12-FB(wt)
Plasmid#202385PurposemApple-Talin(1-2299)-FRBDepositorInsertmApple-Talin(1-2299)-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-TalinN3-FRB(wt)
Plasmid#202384PurposemApple-Talin(1-911)-FRBDepositorInsertmApple-Talin(1-911)-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mApple-TalinN10-FRB
Plasmid#202380PurposemApple-Talin(1-1973)-FRBDepositorInsertmApple-Talin(1-1973)-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF2
Plasmid#200376PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H5_fold-0_Elsa
Plasmid#201825PurposeExpresses H5_fold-0_Elsa in bacterial cellsDepositorInsertH5_fold-0_Elsa
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA28-pMa-PspCas13b crRNA-TS1
Plasmid#199459PurposeU6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )DepositorInsertTS1 crRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA17-CRISPR-Tag (11XTS1) for dSpCas9
Plasmid#199448PurposeCRISPR-Tag sequence that can be efficiently bound by dSpCas9DepositorInsertCRISPR-Tag
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_L172Q_KDEL_pBABEpu
Plasmid#183690PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)DepositorInsertER Halo(L172Q)
UseRetroviralTagsExpressionMutationL72Q in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu
Plasmid#183691PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)DepositorInsertER Halo(M21K_F86L)
UseRetroviralTagsExpressionMutationM21K_F86L in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_Jdomain
Plasmid#183693PurposeBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domainDepositorInsertER Halo(K73T)-J domain
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6-mut-siRNAres_I
Plasmid#146588PurposeMammalian Expression of HsSmg6-mut-siRNAresDepositorInsertHsSmg6-mut-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12Fuzzy3MYB
Plasmid#194440PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
HySp5-310
Plasmid#192995PurposePlasmid that encodes for Hydra Sp5, lacking the SP boxDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP boxPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN1
Plasmid#194439PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1DepositorInsertgRNA targeting CEN1
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1093B
Plasmid#194003PurposepBac-U6-crCHIKV-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting CHIKV
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1085F
Plasmid#191375PurposepBac-U6-crYellow-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting yellow
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC2
Plasmid#191857PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003_sgTFAP4 guide 2
Plasmid#193587PurposeTFAP4 knockoutDepositorInsertsgTFAP4 guide 2 (TFAP4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HySp5-557
Plasmid#193000PurposePlasmid to generate an ISH probe for Hydra Sp5DepositorInsertHydra Sp5
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZfSp5a-377
Plasmid#192997PurposePlasmid that encodes for Zebrafish Sp5DepositorInsertZebrafish Sp5
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only