We narrowed to 10,133 results for: ada

1. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR4_G3BP2

   Plasmid

   #127105

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Gateway shuttling vector

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHLmMBP-8

   Plasmid

   #72347

   Purpose

   Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR26

   Plasmid

   #127372

   Purpose

   Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Promoter

   tight TRE promoter

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR26-CMVconst

   Plasmid

   #127373

   Purpose

   Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Promoter

   CMV enhancer/promoter

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_OT1.0

   Plasmid

   #185386

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-TS2126 RNA Ligase

   Plasmid

   #119941

   Purpose

   Express TS2126 RNA ligase in E. coli

   Depositor

   Takashi Ito

   Article

   Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

   Insert

   TS2126 RNA ligase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti Lifeact-iRFP670 BlastR

   Plasmid

   #84385

   Purpose

   Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   iRFP670

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHLmMBP-11

    Plasmid

    #72349

    Purpose

    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

    Plasmid

    #159809

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. NO MS-Sensor (35)

    Plasmid

    #199783

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    NO MS-Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-SAKS1-Strep-HA

    Plasmid

    #113487

    Purpose

    Expression of human SAKS1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    SAKS1 (UBXN1 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-UBXD4-Strep-HA

    Plasmid

    #113480

    Purpose

    Expression of human UBXD4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD4 (UBXN2A Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-POMP

    Plasmid

    #86765

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human POMP (POMP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL6-H2B-iRFP720-bla

    Plasmid

    #189991

    Purpose

    Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Human histone H2B
    

    Use

    Lentiviral

    Tags

    iRFP720

    Expression

    Mammalian

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Mutation

    G12V

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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