We narrowed to 8,758 results for: app

1. pSEVAb837

   Plasmid

   #217501

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVAb847

   Plasmid

   #217502

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVAb867

   Plasmid

   #217503

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVAb887

   Plasmid

   #217504

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVAb897

   Plasmid

   #217505

   Purpose

   pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

   Depositor

   Nico Claassens

   Article

   van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

   Insert

   superfolder green fluorescent protein (sfGFP)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT-EGFP-V5

   Plasmid

   #240240

   Purpose

   Expression of EGFP-V5 under control of copper-inducible promoter.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   EGFP-V5
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR_HA-spGFP11

   Plasmid

   #240224

   Purpose

   Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

   Plasmid

   #238166

   Purpose

   Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   VPH-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCA595 - pBA904 Puro-T2A-mCherry

   Plasmid

   #238171

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA594 - pBA904 Puro-T2A-BFP

    Plasmid

    #238170

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    Non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMZ103-rbs1-m3

    Plasmid

    #204465

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L125W-A127S mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMZ103-rbs1-m4

    Plasmid

    #204466

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L125W-A127T mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMZ103-rbs1-m5

    Plasmid

    #204467

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L125F-A127G-S129N mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMZ103-rbs1-m6

    Plasmid

    #204468

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L125W-S129N-L130V mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMZ103-rbs1-m7

    Plasmid

    #204469

    Purpose

    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L125W-A127T-L128M mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMZ103-rbs1-m8

    Plasmid

    #204470

    Purpose

    This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

    Inserts

    quorum sensing regulator LasR (lasR )
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LasR L128F-S129W mutation

    Promoter

    In an operon and PLas

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-DEST (JDW 1205)

    Plasmid

    #229821

    Purpose

    A PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-Cas9-FCPF

    Plasmid

    #220132

    Purpose

    Expresses Cas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    Cas9-FCPF (cas9 S. pyogenes)
    

    Use

    Tags

    His and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. iNicSnFR12

    Plasmid

    #227142

    Purpose

    Genetically encoded fluorescent biosensor for nicotine detection

    Depositor

    Henry Lester

    Article

    Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Insert

    iNicSnFR12
    

    Use

    Tags

    6xHis-HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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