We narrowed to 4,657 results for: lum

1. pAPV-mEmerald-DsRed2-ER-5

   Plasmid

   #170681

   Purpose

   Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-mEmerald-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPV-DsRed2-mEmerald-ER-5

   Plasmid

   #170715

   Purpose

   Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-mEmerald-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPV-FLAG-DsRed2-ER-5

   Plasmid

   #170620

   Purpose

   Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-FLAG-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPV-FLAG-DsRed2-ER-5

   Plasmid

   #170621

   Purpose

   Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   EPV-FLAG-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEE22

   Plasmid

   #161787

   Purpose

   Hi-SC2 reporter delivery plasmid

   Depositor

   François-Xavier Barre

   Article

   Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

   Insert

   Hi-SC2 reporter
   

   Use

   Conjugative plasmid to deliver hi-sc2 reporter

   Mutation

   Himar site included MmeI site, tandem loxP sites,…

   Promoter

   n/a

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. JGI_018307

   Plasmid

   #158831

   Purpose

   Rare earth element alcohol dehydrogenases

   Depositor

   Joint Genome Institute

   Article

   Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

   Insert

   Rhodovulum
   

   Use

   Synthetic Biology

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQP-2363

   Plasmid

   #138975

   Purpose

   Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
   

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD-MTS2 (12)

   Plasmid

   #101698

   Purpose

   MTS2 in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
   

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mPapaya1-CytERM-N-17

   Plasmid

   #56352

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

   Depositor

   Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Insert

   CytERM
   

   Tags

   mPapaya1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PAC_E83_LOV_eGFP

    Plasmid

    #231574

    Purpose

    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pER-mScarlet3_N1

    Plasmid

    #189772

    Purpose

    Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    ER-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMU-ERr

    Plasmid

    #61170

    Purpose

    Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtWAK2-mCherry-HDEL
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    April 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1_Floxed-STOP Fluc

    Plasmid

    #122962

    Purpose

    Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_Floxed-STOP Fluc
    

    Use

    Cre/Lox and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ER-GCaMP3

    Plasmid

    #64854

    Purpose

    Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-GCaMP3
    

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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