We narrowed to 5,906 results for: pcDNA 3.1

1. PE-Cas9

   Plasmid

   #174851

   Purpose

   Express PE-Cas9 in mammalian cells

   Depositor

   Wen Xue

   Article

   Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   PE-Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qMaLioffG

   Plasmid

   #212825

   Purpose

   green fluorescent FLIM indicator for endogenous ATP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al bioRxiv 2023.08.29.555275

   Insert

   qMaLioffG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. negMaLionB

   Plasmid

   #113911

   Purpose

   negative control for Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   negMaLionB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.20_mCherry-MS2x4

   Plasmid

   #205535

   Purpose

   Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.11_Gag-MCP_ZF2-P2A-eGFP

   Plasmid

   #205526

   Purpose

   Express HIV Gag-MCP_ZF2-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP_ZF2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD/NL-BH*deltavpu/RT-

   Plasmid

   #136985

   Purpose

   Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

   Depositor

   Jakob Reiser

   Article

   Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

   Insert

   HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   carries D110E mutation in the reverse transcripta…

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRB-myc

   Plasmid

   #20228

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   FRB (MTOR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T2098L point mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. LacI INCENP

   Plasmid

   #45236

   Purpose

   Target INCENP to lac operator sequence

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   LacI, INCENP-Δcen, mCherry (lacI )
   

   Use

   Tags

   LacI (lac repressor protein) and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   t7

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Vh-HA-3xFRB

   Plasmid

   #20223

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   One copy of FRB has a T2098L mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

10. CMV-mito-CitroffRH

    Plasmid

    #158548

    Purpose

    Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TR therapy reporter

    Plasmid

    #179083

    Purpose

    Fluorescent reporter for alternative splicing

    Depositor

    Thurman Wheeler

    Article

    Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

    Inserts

    DsRed1
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin-αv Headpiece M400GC

    Plasmid

    #159637

    Purpose

    Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    alpha v headpiece M400GC
    

    Use

    Tags

    6x His and Strep II

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rtn3c-mEmerald

    Plasmid

    #186963

    Purpose

    Express Rtn3c with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN3C (Rtn3 Mouse)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p176 Grg-1s Myc His

    Plasmid

    #11065

    Depositor

    Ramesh Shivdasani

    Article

    Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

    Insert

    Grg1-S (Tle1 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniCMV-tdTomado-tDeg

    Plasmid

    #185402

    Purpose

    tdTomado-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    tdTomado-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. enOgeuIscB with ωRNA-v2

    Plasmid

    #220939

    Purpose

    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

    Inserts

    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH2.21_mCherry-MS2x6

    Plasmid

    #205536

    Purpose

    Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. NbV5-Rluc8

    Plasmid

    #201480

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    Rluc8-NbV5
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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