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Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Rab10wt
Plasmid#135557PurposeExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.DepositorInsertRab10 (Rab10 Mouse)
UseTags6xHis tag, TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
UseTagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
UseTags6xHis-TEVExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcrF1
Plasmid#89233PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF1
UseTags6xHis-TEV and FLAGExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
UseTagsHis14 and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF1389
Plasmid#104962PurposeBacterial expression plasmid of bdSENP1 proteaseDepositorInsertSENP1
UseTagsHis14 tag and Tev protease cleavage siteExpressionBacterialMutationPromoterlacUV5Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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6CCR (GID4)
Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0679
Plasmid#149689PurposeBacterial expression of SENP_EuH proteaseDepositorInsertSENP_EuH protease
UseTagsHis14-tag and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
ITCH
Plasmid#36197PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH (ITCH Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains HECT domainPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPTM p62 PB1 (1-102)
Plasmid#210427PurposeBacterial expression of MBP-PB1(1-102) with TEV cleavage siteDepositorInsertp62 PB1 (1-102) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
DOT1L
Plasmid#36196PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDOT1L (GeneScript Synthesized) (DOT1L Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains amino acid residues 1-420PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress414_GmPOPB
Plasmid#92234PurposeExpress wild type prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationnonePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNMT3B (3QKJ)
Plasmid#32044PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDNMT3B (DNMT3B Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPWWP domain only (amino acids 293-442)PromoterAvailable sinceFeb. 22, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEMG1 (EMG1 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDR5
Plasmid#36900PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationmutation version of plasmid #60121 3UR4PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits