2,058 results

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4723

Plasmid

#178899

Purpose

Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pmyo-3

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Publication

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Publication

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Expression

Worm

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1838

Plasmid

#154324

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1592

Plasmid

#121059

Purpose

GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC^BioTag::AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

Expression

Worm

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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