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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Publication

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Expression

Worm

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1838

Plasmid

#154324

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Publication

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Gateway Cloning and Synthetic Biology

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-RGG

Plasmid

#124942

Purpose

Expression of SZ1-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET_SZ1-RGG-GFP-RGG

Plasmid

#124937

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab3P::his-24::mNeptune

Plasmid

#124350

Purpose

Nuclear Localized Pan-Neuronal mNeptune

Depositor

Andrew Leifer

Insert

rab-3, mNeptune (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Expression

Worm

Promoter

EFT3

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBT3003

Plasmid

#122537

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Publication

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2::pisy-1 (pisy-1 Synthetic, Nematode)

Expression

Bacterial

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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