1,878 results

1. pJJS003

   Plasmid

   #188326

   Purpose

   PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::mCherry::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. punc-119cR

   Plasmid

   #21596

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119:mCherry (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   mCherry

   Expression

   Worm

   Mutation

   N/A

   Available Since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW4.02

   Plasmid

   #131372

   Purpose

   expression of MBP:PGL-1 in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   pgl-1 (pgl-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWD509

   Plasmid

   #73739

   Purpose

   Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   2xNLS-FLP-D5
   

   Expression

   Worm

   Mutation

   G5D

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Expression

   Worm

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY15

   Plasmid

   #191021

   Purpose

   flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-1 (flp-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. JH04

   Plasmid

   #73513

   Purpose

   mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mRuby2::PH::tbb-2
   

   Tags

   mRuby2

   Promoter

   mex-5

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW2088

   Plasmid

   #154336

   Purpose

   Crispr repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::GFP::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH3377

    Plasmid

    #175286

    Purpose

    Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    daf-16 d/f (daf-16 Nematode)
    

    Expression

    Worm

    Promoter

    Pges-1

    Available Since

    Nov. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Expression

    Worm

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. optGluCl beta Y182F ECFP

    Plasmid

    #15105

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl beta (glc-2 Nematode)
    

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA, Y182F

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIT179

    Plasmid

    #204510

    Purpose

    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

    Depositor

    Yuji Kohara

    Article

    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

    Insert

    csr-1a (C. elegans) (csr-1 Nematode)
    

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR40

    Plasmid

    #69257

    Purpose

    plin-31::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    lin-31

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH4.52

    Plasmid

    #26946

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    histone H2B (his-58 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSR02

    Plasmid

    #69149

    Purpose

    Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEY12

    Plasmid

    #191018

    Purpose

    Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    rab-3 (rab-3 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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