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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT3024

Plasmid

#122542

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_mt::CemOrange2

Tags

MLSLRQSIRFFKPATRTLCSSRTLL

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBT3007

Plasmid

#122538

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS716

Plasmid

#188888

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker and Phsp16.41::Cre

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJW2088

Plasmid

#154336

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Expression

Worm

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4.52

Plasmid

#26946

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

histone H2B (his-58 Nematode)

Tags

GFP

Expression

Worm

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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SNF-5 pXOOM

Plasmid

#48697

Purpose

Expressed SNF-5 in Xenopus oocytes or mammalian cells

Depositor

Dmitri Boudko

Article

Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

Insert

snf-5 (snf-5 Nematode)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

K42E (when compared to NP_496735.1)

Promoter

T7

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Expression

Worm

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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TC646

Plasmid

#164892

Purpose

Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

GFP

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEY48

Plasmid

#191052

Purpose

klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

klp-6 (klp-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY47

Plasmid

#191051

Purpose

gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-21 (gcy-21 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRG5271Neo

Plasmid

#26387

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pJH3377

Plasmid

#175286

Purpose

Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16 d/f (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBT3037

Plasmid

#122544

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNM3813

Plasmid

#154121

Purpose

mec-4 promoter driving lexA-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::lexA-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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